ORGANISATION/COMPANYInstitute of Plant Molecular Biology, Biology Centre of the Academy of Sciences of the Czech Republic
RESEARCH FIELDBiological sciences › BiologyChemistry › Biochemistry
RESEARCHER PROFILEFirst Stage Researcher (R1)Recognised Researcher (R2)Established Researcher (R3)Leading Researcher (R4)
APPLICATION DEADLINE20/08/2019 00:00 - Europe/Brussels
TYPE OF CONTRACTTemporary
OFFER STARTING DATE01/10/2019
Research project: Trace elements are essential for plant growth and development, and for plant-environment interactions. They are involved in plant metabolism as enzyme cofactors (e.g. Cu, Fe, Zn, Mn), in redox reactions and electron transport (Cu, Fe, Mn), gene expression and transcription factors (Zn). The project aims to elucidate the mechanisms of metal-regulated plant responses to biotic stress such as fungal pathogens using different model plants. To achieve this, plants will be grown under metal deficient (Zn, Mn, Cu, Ni, Fe), optimal and metal excess conditions and exposed to pathogens under simulated environmental conditions. Currently, two model systems are established: Glycine max- hemibiotroph Phomopsis longicolla and Capsicum annuum-necrotoroph Botrytis cinerea. Fast responses to the pathogens will be monitored in vivo by measurements of element distribution and chlorophyll fluorescence kinetics by imaging techniques. Metabolome, metalloproteome and transcriptome profiling will be performed on harvested samples. Finally, candidate metal-regulated genes which promote plant immunity should be proposed.
The prospective PhD student will be able to learn various genetics, molecular biology and plant physiology techniques including metalloproteomic analysis by HPLC-DAD/ICP-MS, qPCR, QISH, microscopy techniques, chlorophyll fluorescence kinetics, and analysis of antioxidative metabolism.
We are looking for a motivated student interested in multidisciplinary plant sciences including experimental and bioinformatic approaches. Qualified candidates holding or about to obtain a Msc or equivalent degree in Plant Biology, Biochemistry, Bioinformatics, Microbiology or related field are encouraged to apply. Previous experience in biochemistry and molecular biology is an advantage. Furthermore, the candidate should have some theoretical knowledge and practical experience in at least one of the following areas: plant physiology, molecular biology, biochemistry, bioinformatics. The candidate should have a good knowledge of spoken and written English to be able to fluently communicate with the team and to write scientific manuscripts for publication in international journals. Good communication skills and team work abilities are required.
EURAXESS offer ID: 417931
Posting organisation offer ID: 86142
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